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The process was repeated for a third passage.
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The optical pumping beam is retro-reflected by a dichroic optical mirror (item 7 of Figure 2) for a second passage through the cell.
This step was repeated for a second passage.
After reading three passages, each student took a cued-recall test for one passage, a multiple-choice test for a second passage, and no test for the third passage.
The allantois from the eggs where embryonic death was observed within a week was used for a second passage in embryonated eggs, in addition to the performance of an HA test.
Nasal swab and lung samples were considered negative for IAV if a second passage in specific pathogen free chicken eggs or in MDCK cells was negative by HA test and RT-PCR.
For these 2 isolates, a second passage was performed in Tg110 mice by using a brain homogenate derived from a mouse with either H-type or C-like PrPres.
Because the virulence of the first-generation rabbit HEV was limited, we used virus recovered from the feces of rabbits inoculated with the GDC54 strain of HEV as a second passage inoculum for the further studies.
If IFA results were negative for dengue viruses on first passage, a second passage was made, and cells were again harvested on days 5 and 10 for IFA.
Because a fourth passage was not performed for Chinese hamsters, whether third passage reflects the stable incubation period is unknown.
But late tonight, the measure was withdrawn after sponsors realized they did not have the 24 votes for a three-fifths passage.
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