To meet the criteria for a suitable reference gene, the transcript must be detected in all organs, developmental conditions and under a variety of stress conditions.
Hughes (1995) summarized the basic characteristics necessary for a suitable reference condition including reasonableness and political acceptability, sufficient number of reference sites within the area of interest, and suitable data on natural conditions of the site.
Since the publicly available genomic data [SRA: SRP019803] was of insufficient quality for the generation of a suitable reference sequence, we mapped the emu transcriptomic reads to the ostrich stk33 mRNA sequence and extracted the consensus sequence.
The present study was limited by its observational design; the lack of a suitable reference for SCL-90R psychological dimension norms for the Liberian population; and the small study sample size which might have precluded finding significant associations among variables.
The choice of a suitable reference electrode for electrochemical testing of metals in organic solutions poses a major difficulty.
However, for populations that are phylogenetically distant from the samples present in the reference panels, the selection of a suitable reference panel for imputation becomes less clear.
In this paper, we use genotypes imputed based on population wide LD, offering a strategy that can be applied universally in any population, for which a suitable reference panel can be assembled.
In constructing questionnaire items for 'motivation', Pintrich et al.'s (1991) Motivation Strategies for Learning Questionnaire (MSLQ) was considered a suitable reference for the present study, because it is widely applicable in the field of educational psychology, and aimed at university students.
The results showed that the median nucleotide coverage for the 6159 genes within the ALHF transcriptome was >99% for all of the M. domestica strains tested, demonstrating that the transcriptome from ALHF was indeed a suitable reference for the determination of gene expression levels for all the strains.
Since the dysplastic femur presents abnormal neck anteversion, in this series we did not consider the femoral neck to be a suitable reference for stem placement, therefore the correct orientation of the stem was determined referring to the condylar plane.
The Perkins tonometer (PAT), as the handheld version of GAT, is a suitable reference standard for IOP measurements for patients in the supine position, and hence for children during an examination under anaesthesia.
