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An additional challenge for disseminating the database has been the requirement to perform a detailed phantom calibration to define normal ranges for a specific imaging system [10].
It is relatively straightforward to control these parameters to tune the LSPR properties of gold nanoparticles for a specific imaging modality.
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Therefore, we evaluated the effectiveness of the activatable system in MT1-MMP-expressing tumors and the potential of MT1-hIC7L as a specific imaging probe for MT1-MMP-expressing tumors using in vitro cellular uptake assays and in vivo imaging studies.
However, the iterative thresholding method (ITM) requires a regression curve which has to be determined by phantom measurements for every specific imaging setting [13].
However, localization based on structural abnormalities resulted in several false positives, highlighting the need for more specific imaging agents.
These properties have been widely utilized in biology and biomedicine, for instance, for molecule specific imaging and for the diagnosis of diseases like cancer, for photothermal therapy, or for real-time optical sensing with zeptomole sensitivity.
Compared to other techniques used for pigment specific imaging such as (auto fluorescence imaging, PS-OCT enables depth-resolved imaging rather than integrating signals from all depths for one transverse position.
These analyses typically exploit carefully optimized protocols for specific imaging targets.
Studies are ongoing in defining valid paradigms for specific imaging and/or quantitative applications of the DH methods in biology: this requires efforts in comparative studies using a strict interdisciplinar cooperation between experts in life-science and optics.
Advanced coatings may prove useful for specific imaging applications like 2-photon [ 27] or autofluorescence [ 28] where light efficiency is paramount.
This approach would only be required when using methods that cannot be calibrated for specific imaging parameters, e.g. threshold-based methods, either with or without background corrections, in order to ensure inter-institute comparability of PET-based tumour volume assessments.
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