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A possible implication of this result is that even for a single section system the above result may not hold in all the cases.
Parameters for a single section converged rapidly as the number of traverse lines increased, although unacceptable variations were in evidence across multiple sections.
Using three hardened cement paste specimens composed of different air-void systems, we performed sensitivity analyses in terms of the number of traverse lines employed for a single section and the number of sampling sections across the height of a specimen.
Movie 1 available as supplementary on-line material shows the progress of the optimization for a single section dataset consisting of 6 × 6 tiles.
Movie 2 shows the progress of the optimization on three serial sections imaged as 4 × 4 tile mosaics demonstrating that the procedure for multiple section is essentially the same as for a single section.
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A subset of sections was first immunostained for OC and visualization with Alexa Fluor 568 and then counterstained with thioflavin S. Immunostaining by the OC antibody was captured from a single section for each AD, DS and control case using a 20× objective, a Sony high-resolution CCD video camera (XC-77) and the built-in video capture capabilities of a Macintosh 8100/80AV.
Three nonadjacent sections from each animal were analysed for PGP 9.5; a further three separate sections for GAP-43 (for paw pad and back skin); and a single section for CGRP, SP, and β-tubulin (for paw pad skin only).
TESS will monitor thousands of stars simultaneously for such "transits," watching a single section of sky for a month straight before moving on to another.
To estimate the reduction of normal-looking neurons, we counted the number of positive cells for the different LSG in a single section for each sample using Image Pro Plus 7.0 software (Media Cybernetics, Bethesta, MD, USA).
For the image analysis, only a single section, which corresponded to a section at almost the same level relative to the upper and lower extremities, was used.
Blackhurst said the aim is for a single-section "faster, more accessible and urgent paper".
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