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The studies were each for a single locality, had small sample sizes, focused mainly on direct costs, and did not take into account the impact on families or estimate the injury burden.
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In three cases there was a reasonable sample size for specimens from a single locality and habitat; therefore, we tested for an age affect using these groups.
Because the inclusion of localities with a small number of strains may bias the result of AMOVA, we included only strains for which more than five isolates were available from a single locality.
Our sample sizes for some species were rather low, particularly for C. thyrorus (N = 2) and C. jariina (N = 9), each from a single locality.
The most restricted is A. cilicicus, which is known only from a single locality in southern Turkey.
For example, it occurs widely, though localized, in Aragón, where Myotis sp. A (the only other species in the M. nattereri complex to occur there) is known from a single locality only.
Individuals were collected from a single locality in Riverside, California (USA), live frozen in liquid nitrogen, and stored at −80°C.
These π values probably are underestimates, however, because each was obtained from a single locality.
P. inaequipes is known from a single locality in southern Corsica (Bonifacio).
Each major clade, and several subclades contained localities from multiple drainage basins (with the exception of Western Great Basin which only contained individuals from a single locality).
All animals in this study originate from a single locality just outside Brazzaville, the capital of the Republic of Congo.
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