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The measurements were carried out at constant temperature of 37 °C, and the results, for a selected concentration (0.05 mg/ml).
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The linearity studies were carried out by 6 point calibration method with triplicate analysis at each selected concentration for all three standard compounds.
Powder-like nanocarbon had weighed out for selected concentration and added to epoxy acetone solution.
The change in pH was found to be shifting toward the acidic range for all the selected concentrations (Fig. 1c).
The initial values of BOD was almost the same for all the selected concentrations as the microorganisms inoculated into the minimal medium entered the lag phase due to the exposure to high concentration of compound (Fig. 1b).
The same procedure was performed for the main study, using the two selected concentrations.
These MICs were performed for the purpose of selecting concentrations of these agents that have no significant effect on the growth of the strains used.
The effects caused by dry spiking were concentration-dependent for the selected test concentrations (3-6 concentrations, depending on the test) whereas there was no such relationship for the two test concentrations when wet spiking methods were applied using water as the carrier.
A wavelength of 427 nm was selected for concentration determinations.
The basis for selecting the concentration levels for each of the parameters under consideration in the above classes is detailed below.
We selected this concentration for mini-chaperones because other studies have shown that αA-mini-chaperone exerts protection against oxidative injury to RPE cells at this concentration.
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