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A single tumor-reactive antigen-specific CTL clone expanded to 1010 cells/m2 is used for both a first infusion given to patients without conditioning, and then, for a second infusion, following patient conditioning.
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Patients experiencing satisfactory results may expect significant relief from a second infusion for recurrent pain.
A second infusion was administered 24 hours later.
Responses on the active lever (counterbalanced left/right between animals) resulted in house lights off and drug-paired lever cue light on for a 3-second infusion (0.015 mg/kg nicotine in 0.050 ml saline; FR1 schedule).
However, one limitation of this study is that we cannot fully rule out the possibility of a sub-detectable population from the first T cell infusion involved in altering the immunologic milieu for the second infusion.
The postinfusion observation period for the second infusion was shortened to 30 min and was withheld over subsequent administrations provided no adverse events occurred.
Time from start of infusion might also have confounded some series; by randomly assigning patients to begin their first cycle at either 6 a.m. or 6 p.m. and then alternating for the second infusion, we were able to adjust for the steady rise in 5-FU levels that was observed with the higher dose infusions.
Group 9 (Losartan/losartan+L-NAME) (n = 7): During the generation of the baroreflex curves in this group, losartan (7.5 μg kg−1 min−1) was infused as in the first I.C.V. infusion and then losartan plus L-NAME (7.5 + 150 μg kg−1 min−1) for the second infusion before the production of the baroreflex curves.
One infliximab patient had myalgia for a few days after the second infusion and another infliximab patient had a bad taste in the mouth for a few days; both events were considered unrelated to the drug by the safety review committee.
We also found that mean 5-FU concentrations were higher during the second infusion a week later for the same patient, although only by 8%; higher 5-FU concentrations during a subsequent cycle were also observed by Kuwahara et al [ 12]. White blood cells, which have the highest level of DPD expression, are known to have a diurnal variation in number [ 27].
In the combined experiment, the higher sampling intensity (every 5 minutes) was reinstituted for 60 minutes when the second infusion started.
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