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In order to determine whether CLE peptide binding would have an effect in planta twelve plants were used for a root dip assay.
In planta assays used twelve plants of each of cultivar X5 and Westag 97 in two separate experiments over a month for a root dip assay [ 55].
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To minimize any environmental impacts, we grew the cotton seedlings in sterile culture pots and used a root dip-inoculation system for introducing the fungus.
For all greenhouse infestation experiments, a root-dip inoculation procedure [ 31] was applied which was modified for A. thaliana as follows.
DAL carried out the root dip assays.
In this study, we established a laboratory unimpaired root dip-inoculation method for inoculation and found that it accurately reflected the progress of a natural infection of cotton by V. dahliae V592 (Figure 2A).
For a root cutting, transplant directly.
We established a laboratory unimpaired root dip-inoculation method to detect insertional mutants with altered pathogenicity in cotton.
For root-dip inoculations, a conidial suspension (10 mL-1) was prepared.
The root dipping for all the cultivars was done using each isolate separately.
Seedlings of the mapping parents (G. hirsutum cv. Emian22 and G. barbadense acc. 3 79) with two euphyllas were inoculated with the V991 strain of V. dahliae by root dipping for 1 minute.
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