Total RNA was used for cDNA synthesis using a Revert Aid M-MuLV Reverse transcriptase (Fermentas, Vilnius, Lithuania) according to the manufacturer's recommendations.
Asked how, in her case, an actress prepares, she doesn't for a moment revert to the pretentious twaddle that sometimes comes out of the pretty mouths of her contemporaries.
The most likely time for a human to make a revert is either within two minutes after a change has been made, after 24 hours, or after a year.
The threshold model thus supports an environmental Kuznets curve for deforestation that lacks a reverting trend for richer economies.
The Arabic maqām, roughly equivalent to the rāga, provides the basis, among others, for the bashraf, a reverting type not unlike the Western rondo, and the nawba, an extended multisectional performance with some resemblance to the suite.
A two-year 0.25% increase in all rates expires Dec. 31, 2010, so the rate on income over $94,111 for a married couple and $47,055 for a single reverts to 9.3%.
Her husband, Andrew, an advertising sales representative, complied for a while, then reverted to normal hours.
In Glendora, a town that calls itself the Pride of the Foothills, the whipping flames were clearly visible on the hills to the north of Route 66, a thoroughfare renamed Alosta Avenue for a time before reverting to its original, legendary title.
And so, for a while, I reverted back to the shadows.
Other than when we tried to conceive and while I nursed (our daughter, for 18 months, our son, for a year), we reverted to the tried-and-true pull-out method, our dear, dear friend.
