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DNA was amplified by polymerase chain reaction (PCR) using primers specific for a repetitive element (RLEP) characteristic of M. leprae.
Conversely, if the contig represented a consensus sequence for a repetitive element within the source (meta genome, such normalization would appropriately distribute the evidence scores across the multiple loci to which that evidence had been applied, making it easier for locally-anchored read evidence to override the consensus sequence.
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These estimates are conservative because more repetitive elements are expected to be identified if the criteria for defining a repetitive element family are less stringent.
All MRSA specimens from community hospitals and selected OSU MRSA blood isolates and isolates from patients residing in the catchment areas of the outreach hospitals were prospectively collected during March 2009 February 2010 for genotyping by using a repetitive element PCR (rep-PCR).
DNA content was normalized to that of long interspersed element 1 (LINE1), a repetitive element for which copy number per haploid genome is similar in both the normal DNA sample and DNA from cancer cells.
DNA content was normalised to that of long interspersed elements (LINE1), a repetitive element for which copy number per haploid genome is similar in both the normal DNA sample and DNA from cancer cells.
Briefly, we have quantified each tumor DNA by comparing the target locus to the reference Line-1, a repetitive element for which copy numbers per haploid genome are similar among all normal and neoplastic human cells [ 19, 20].
All ChIP-seq and RNA-seq data were processed with RepEnrich to generate counts for all repetitive element subfamilies.
RepEnrich also allows for a custom repetitive element annotation, which can be used for a variety of applications where multi-mapping reads become an issue such as gene clusters repeats that appear in tandem duplicates.
EdgeR analysis yielded the log2 fold changes for ChIP with respect to input and an associated p-value for each repetitive element subfamily.
Notably, hybrid formation in trans might be a more potent promoter of genome instability than hybrid formation in cis, particularly for hybrid RNA generated from a highly repetitive element, which in trans can cause instability at a plethora of targets.
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