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These experiments reveal a novel role for a Ras superfamily member in catalyzing cyclin E turnover during S phase, as well as an unexpected, essential role for the Golgi as a ubiquitylation platform for cell cycle control.
Forty-eight hours after transfection, cells were harvested for a Ras activation assay.
For a Ras activation-redistribution assay, COS-7 cells cultured on glass bottom dishes from Mattek Corp. (Ashland, MA) were transiently transfected with YFP-RBD and serum starved for 16 h, after which they were stimulated with the indicated compounds.
This complex organisation of the RHOH 5' region is unusual for a Ras related gene and is reminiscent of cytokine or cytokine receptor genes.
A low score for a RAS signature generated in silico from three publicly available RAS pathway profiles (Sweet-Cordero et al, 2005; Bild et al, 2006; Blum et al, 2007) correlated with sensitivity to MK-2206 in a panel of breast cancer cell lines (Loboda et al, 2010).
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The chloride diffusion coefficient of the concrete increases with an increase of D om for a given A ra.
The value of D RAC increases with an increase of the R rm for a given A ra.
Capacity for a RA system with one and two RNs with single-antenna terminals was studied in [16].
When R rm increases from 0 to 0.5, D RAC increases by 50, 133, and 300 % for A ra values of 0.2, 0.4, and 0.6, respectively.
When D om increases from 3 to 9 (×10–6 mm2/s), D RAC increases by 21, 47, and 80%% for A ra values of 0.2, 0.4, and 0.6, respectively.
Another interesting gene linking autophagy and ovarian cancer is the aplasia ras-homolog member 1 (also; also known as DIRAS3), which codes for a ras-homolog 26 kDa GTPase.
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