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This choice of markers thus probably selected for a minor population of cells.
For a minor population that was observed to form colonies, they failed to progress further to become SSEA1/NANOG+, as shown in new Figure 7C.
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An illustration for a tumorigenic expansion of a minor population is the finding that in "luminal -type Wap-Cre;EN mammary carcinomas [24] and luminal -type-T tumors (this study), the embryonic K8+K14+ cellular subset [53] is significantly expanded, Wap-Cre ENhe adult normal mammary gland K8+K14+ cells constitute a rare population that overlaps Wap-Cre EN lumammaryrogenitors [24].
The presence of a minor population of cells positive only for PI indicates that PSY also induced necrotic cell death.
Since presumably only the DNA of a small percentage of complexes reaches the nucleus for transcription a minor population of complexes undetectable by biophysical methods might actually be the biologically active one leading to transfection.
Recent studies have shown that Arf proteins can act in synergy to produce an activation cascade, as shown previously for Rabs [88], with a minor population of GTP-bound activated Arf6 or Arl4 binding to the PH domain of the Arno GEF to enhance binding of Arf1 to the Sec7 domain, thus triggering Arf1 activation [89].
Taken together, these results indicate that for most sera anti-GAD67 represents a minor population of anti-GAD65 reactive with an epitope shared between GAD65 and GAD67.
Therefore, a minor population of lymphocytes that required RAG1 for development played a critical role in suppressing disease.
Normal mesothelial cells, that was a minor population of the biopsies (Table 3) stained positive for NQO1 and VG5Q, weakly for RAD21.
Spheroids also possess a minor population of cells that are mesodermal in origin staining positive for Vimentin protein (VIM).
A minor population of bone marrow-derived mesenchymal stem cells has also been found to be positive for 3G5 [ 20].
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