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In this study, unique genes with a p1 t) > 0.999 for a minimum of two time points and absolute value fold change ≥ 1.5-fold compared to time-matched vehicle control for at least one time point were considered differentially expressed.
Model-based t-values that compared treated and vehicle responses on a per time-point basis followed by Empirical Bayes analysis identified 1,206 differentially expressed genes across the time course based on a p1 t) > 0.999 for a minimum of two time points and absolute fold change ≥ 1.5-fold compared to time-matched vehicle control for at least one time point (Fig. 2A).
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Outcomes were assessed at a minimum of two time points.
Each experimental condition was performed a minimum of two times.
In vivo experiments were performed a minimum of two times.
In vitro data represented a minimum of two to three independent observations and were performed a minimum of two times.
Supplement data was collected a minimum of two times, and maximum of three times during pregnancy.
Each experiment was repeated for a minimum of three times.
The analysis was repeated for a minimum of three times using independent cultures.
All experiments were repeated for a minimum of three times with each experiment done in four replicates.
The entire test was repeated for a minimum of three times.
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