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Images were projected through the 1.52 µm Z stacks for a maximum intensity value and quantified using the Metamorph software with a set threshold value, calculating the average signal intensity per cell.
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The thin sections can be used for detailed evaluation; a maximum intensity projection is useful for overview of the urinary tract.
Then, the 200-μm slice was immunolabeled for NeuN, and a maximum intensity projection of confocal NeuN fluorescence image stacks was acquired from the upper 15 μm of the slice (measured perpendicular to the slice plane, see Supplementary Fig. 1 B ).
The maxima at 700 and 800 nm keep roughly the same intensity for each polarization direction, whereas the peak at 900 nm reaches a maximum intensity for 0° polarization and a minimum for 80° polarization.
The peak at 700 nm shows a maximum intensity for 80° polarization and a minimal intensity for 0° polarization.
For single-molecule FISH images, a maximum intensity projection of Z stacks was used to quantify mRNA distribution.
2) It is stated that an average crystal shows a maximum intensity for the strongest spot of 8.4 x 10**-3 of the direct beam.
The color of the whole plot is implemented to have a maximum intensity for absolute values greater or equal to eight.
Using a method similar to that described earlier for Figure 2, we constructed a maximum intensity 3D projection of the time-lapse video sequences (Supplementary Video 3), using the Image J RGB Zprojector and JaCoP plugin.
For example, considering SNOM signal has a maximum intensity when the tip approaches close to a sample surface in DFM mode, the other signal should have a maximum intensity when the tip separates from the sample surface.
Middle, a maximum intensity projection for direct excitation (red) and inhibition (blue).
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