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The working hypothesis for a retreat measurement system was based on discriminating the highly reflective rib-wall bolt-plates from the background coal, and using these features as reference waypoints for a localisation solution.
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In this context, the localisation pattern of the almond 9-HPL differs significantly from the cytosolic distribution of HPLF and this is the first report showing such a localisation for HPL.
The strong directional dependency of the dark-field signal allows for a precise localisation of the objects even in projection.
Finally, TargetP shows a strong prediction for a mitochondrial localisation, but targeting signals are often non-conserved in Trypanosomatids [[ 18]; our unpublished data].
The requirement for a cytoplasmic localisation for the anti-apoptotic effect of Bag-1 was demonstrated by the overexpression of Bag-1S tagged with a nuclear localisation sequence (localised entirely in the nucleus) which failed to protect against apoptosis (Townsend et al, 2004).
Sequence analysis of the human PBGD protein revealed RR X5 RK X17 RK repeats between residues 149 and 180, which may fulfil the requirements for a nuclear localisation signal.
Raw MaxQuant output files were subjected to the following filtering criteria prior to their inclusion in further bioinformatics analysis: (1) contaminants and reversed matches were removed, ensuring that all identifications were reported with an FDR of <1%; and (2) pY sites were filtered for a minimum localisation probability confidence of 0.75.
In the DMD cells the nuclear rim staining was retained for Emerin and Lamin and a localisation of Emerin and LaminA/C in blebs and protrusions was observed in dysmorphic nuclei (Fig. 1a, arrow).
This paper introduces a proposal for an indoor localisation and orientation distributed service built on a dynamically reconfigurable platform.
In our settings, non mitochondrial Bcl-2 can hardly account for an ER localisation (Fig. 5B); further experiments are required to identify the host compartment and characterise the molecular basis for such pro-autophagic functions.
It matters for example whether the motif is a targeting signal for subcellular protein localisation, a degron for protein destruction, or a protease cleavage site, so design accordingly.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com