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A novel active element for a humidity sensor based on Ca2+-doped PbTiO3 perovskite ceramic film has been prepared by the sol gel technique.
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For instance, a humidity sensor using a tubular microcavity as a core component can be fabricated to detect the humidity variation of the environment.
For the model, a humidity variable with a 4-month lag and a precipitation variable with a 3-month lag were selected.
Hemolymph samples were allowed to settle for 30 min in a humidity chamber, fixed for 5 min with 4% paraformaldehyde, and incubated in 0.1% TritonX-PBS including Alexa Fluor 594-conjugated phalloidin and DAPI.
Ligation reactions, were incubated under coverslip at 37°C for 30 min in a humidity chamber, washed in wash buffer for 3 min at room temperature, dehydrated and air-dried.
For hybridization, the arrays were incubated at 42°C for 12 h in a humidity chamber.
The sample was air-dried and conditioned at 80% RH for 30 minutes in a humidity chamber and then flattened using blotters, felts, and weights.
at room temperature for 10 min in a humidity chamber.
Non-specific binding was blocked by incubating the slides in normal rabbit serum for 20 min in a humidity chamber.
Slides were incubated in 4 drops per section of Image-It FX Signal Enhancer (Invitrogen) for 30 min in a humidity chamber and then rinsed with PBS.
After washing with water, the slides were hybridized with 600 nM CCAT1 probe at 55°C for 90 min in a humidity chamber.
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