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When the precision estimates have been improved with other data sets it will be possible to establish guidelines on how low expression levels of mRNA transcripts are detectable in the original sample for a given sample purity, and thus, give advice on the detection abilities of e.g. low-abundant transcription factors and stem cell genes.
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The purity fold improvement of a component calculated for a given sample (AWF, RF, or WHE) was calculated as the ratio of its specific yield relative to the specific yield in the total.
For the remaining samples, purity and concentration were determined using spectrophotometry (Ultrospec 2000, Pharmacia Biotech, Bucks, UK).
The thermal conductivity of pure aluminium at cryogenic temperatures varies by many orders of magnitude depending on purity and treatment, and there is little information in the literature on the likely values to be obtained for samples of a given purity.
Given the purity and performance of the sample, no further purification procedures (FLAG tag, ion exchange, etc).
Age calculations, including correction factors for fractionation and sample purity, are also discussed.
We show that an initial bead-based normalisation step can remove the need for quantification and improves sample purity.
RNA sample purity (A260/280 and A260/230 ratios) will be reported for each sample.
Gives samples of these.
First, 171 potential lead peptides were screened for acceptable sample purity using MALDI-MS, those with purity less than 70% were discarded.
For instance, external estimates for a lower bound on the sample purity could be used.
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