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As previously observed [ 16, 17], the kinetic signatures for a given modification varied slightly depending on the surrounding sequence context.
In these cases, the relative abundances of the MS/MS fragment ions characteristic for a given modification site were used to calculate the DMBNHS modification rate constant.
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For a given histone modification type, the histone modification variation score (HMV) for a given TF in each of the 4 blood cell lineages was calculated using the formula: Ci represents a specific blood cell lineage and is characterized by discrete values for the three histone marks.
Histone modifications can be studied using chromatin immunoprecipitation (ChIP) with antibodies specific for a given histone modification.
For a given histone modification type, each TF was represented by a vector of 4 numerical values representing the histone modification levels of the 4 blood cell lineages.
We identified a high degree of variability in the marking repertoire of each cell type – with one or two cell types showing exon bias for a given histone modification, while the third cell type showing the opposite (i.e., intronic) or no exon-intron bias at all.
For example, after ChIP-seq data for a given histone modification ("mark") is generated, one might ask: 1.
However, with this in mind, it is important to note that the elucidation of a specific physiological role for a given glycan modification, such as sialylation, poses formidable challenge.
We compared gene expression levels between genes possessing at least one region significantly enriched for a given histone modification and/or DNA methylation in order to evaluate the redundancy of DNA methylation to individual hPTMs in explaining gene expression levels.
We found that, among genes possessing at least one region significantly enriched for a given histone modification, those with DNA methylation exhibited consistently higher expression levels and consistently lower expression bias than those with the same modifications but no DNA methylation (fig. 2 and supplementary fig. S4, Supplementary Material online).
A composite primary key of both 'PARENT' and 'CHILD' fields ensures that the data in this table is non-redundant with respect to the connectivity mapping for a given composite Standard InChI. Figure 1 Modifications to the UniChem schema required to implement connectivity search.
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