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That advice works for a fume hood of any age.
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Like most Cubans, he had no car; he biked or walked barefoot, or waited for a fume-spilling bus with that patience and stoicism that calms down stress.
Samples were left to dry for 10' in a fume hood before addition of 50ul nuclease-free water.
Slides were dried for 1h in a fume hood, cleared in acetone for 2min then rinsed in phosphate buffered saline (PBS)/0.1% Triton X-100 (PBST).
The organic solvent was then evaporated at room temperature for 6 h in a fume hood.
The solvent was evaporated under a gentle stream of nitrogen and the vials were left to polymerise for 72 h in a fume hood.
Five milliliter of concentrated H2SO4 was added and the mixture was allowed to digest for 1 h in a fume cupboard by gentle heating until the vigorous effervescence subsided to ensure that the digest was free of charred organic matter.
The chloroform was then evaporated using a stream of nitrogen for 15 min in a fume hood.
Cells were grown in LB to an OD600 of 0.5 at which time LB plates (0.7% agar) dried for 20 min in a fume hood (face up) where inoculated with 5 µL culture and dried an additional 10 minutes.
Scaffolds were produced and left to dry for 1 h in a fume hood.
The casted solution was left overnight in an acetone closed chamber and dried for 1 day under a fume hood.
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