Sentence examples for for a final extension from inspiring English sources

The PCR conditions was as follows: an initial denaturation at 95°C for 5 min, followed by 35 cycles of 95°C for 30 s, 62°C for 30 s and 72°C for 90 s; and finally 72°C for 10 min for a final extension.

Subsequently, the institute director asked the parish priest for a final extension of the removal date by two weeks Mosspetspromproyekt vacated the building on 13 January 1996.

The reaction was continued for a final extension at 72°C for 10 min. Normalization was carried out simultaneously by amplification of EF-1α using an antisense primer 5'-CTGGCTGTAAGGTGGCTCAG-3' and a sense primer 5'-CATGTCGATTCTGGCAAGTC-3' under the same conditions described above.

The reaction cycling conditions were 94°C for 1 min, then 3 cycles of 94°C for 30 s, 64°C for 30 s, 72°C for 90 s, then 72°C for a final extension.

The PCR amplification program started at 5 min at 95°C, followed by 32 cycles of 30 s at 95°C, 30 s at 60°C, and 1 min at 72°C, and then 10 min at 72°C for a final extension.

PCR reactions were carried out in GeneAmp PCR System 9700 (Applied Biosystems, USA) under the following conditions: 5 min at 94°C, followed by 35 cycles of 1 min at 94°C, 1 min at a primer-specific temperature (Additional file 2, Table S2), and 1 min at 72°C and, for a final extension, 5 min at 72°C.

PCR reaction consisted of initial denaturation at 94 °C for 1 min, followed by 30 cycles consisting of 94 °C for 30 s for denaturation, 56 °C for 30 s for annealing and 72 °C for 30 s for extension, a final extension at 68 °C for 7 min with final hold at 4 °C.

The PCR cycling conditions were as follows: initial denaturation at 94°C for 2 min, followed by 40 cycles of: 94°C for 30", 60°C for 30", 72°C for 2', with a final extension at 72°C for 10'.

Cycle conditions were set up as an initial denaturation step at 92°C for 2′, 40 cycles of denaturation at 92°C for 10′, annealing at 48-52°C 48-52°C, and extension at 68°C for 10', and a final extension step of 68°C for 8′.

The PCR conditions used were: 95 °C denaturation for 2 min, amplification reaction for 33 cycles (95 °C for 20s, 55 °C for 20s, 72 °C for 40s), with a final extension step of 72 °C for 5 min.

Reaction conditions were as above, with a cycle consisting of an initial denaturation of 94°C for 5', followed by 35 cycles of 94°C for 45″, 50°C for 1' and 72°C for 1' 30″, and a final extension of 72°C for 10'.