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The technique often accused of being brutal is the "cry it out" approach – invented by Dr Emmett Holt in 1895, which involves letting your baby cry for a defined amount of time before comforting them.
Not only prenatal but also postnatal stress, for example, induced through maternal separation of the pups from their mother for a defined amount of time (e.g., 2 4 h per day for up to 3 weeks) can result in long-lasting behavioral alterations.
The sample is then ground for a defined amount of time, and the vial is then declogged and pierced.
After agreement has been reached on specific goals, innovations which have not yet been included in publicly-financed healthcare are financed by a public fund for innovations for a defined amount of time.
For all figures except Figure 4A,B, 5, and 8, test food were prepared by seeding 10 μl of bacterial culture in LB (OD = 5.0) on new NGM plates and incubating the seeded plates at RT for a defined amount of time (5.5 hr for HB101, JU54 and PA14 pstP and 7 hr for DA1878).
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At desired time intervals (one time after 30 min of electrospinning for obtaining 1 1 ratio, and two times every 20 min for 1 2 ratio) a defined amount of coral microparticles was loaded on the collected nanofibrous mat (Fig. 1).
In that case, a borrower may win a lawsuit only by showing that the disputed mortgage lacked the precise features required for a qualified mortgage, like a defined amount for points and fees.
A series of 28 3-dimensional image stacks were quantitatively analyzed (see Methods) for the significance of colocalization as a defined amount of pixel overlap (Figure 8A).
For each single specimen, an individual spacer was created using a defined amount of 10 ml bone cement.
The resultant supernatants were recovered, a sample of defined volume (5µl) was dried and weighed, and a defined amount of each sample was transferred to a clean glass vial either for further processing or for MRM-MS analysis.
After pipetting of these components was finished, the plate was transferred to the plate-reader using the robotic gripper and incubated there for 10 minutes in order to enable temperature equilibration to 37°C at which the assay was performed and to expose the enzymes to the final conditions for a defined minimum amount of time.
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CEO of Professional Science Editing for Scientists @ prosciediting.com