Exact(22)
ApoE deficient mice (C57BL/6 background) were purchased from Jackson Laboratories (Bar Harbor, ME), mated with PDZK1 deficient mice (129 SvEv background), and backcrossed for 6 generations into C57BL/6 background to generate the PDZK1/apoE double knockout (dKO) and apoE single KO mice as previously described [21].
Balb/c AID−/− mice were generated by backcrossing AID−/− mice (Muramatsu et al, 2000) for 6 generations by speed congenics (Ramiro et al, 2004).
Mice were backcrossed to C57BL/6 for 6 generations.
After culturing for 6 generations, cells were ready for use for experiments.
My family has been here for 6 generations.
To eliminate any behavioral effects caused by the y−w− background, flies were backcrossed to w1118(CS) line for 6 generations using the scheme described earlier [67].
Similar(38)
B6 129P2- Irf7tmiceg mice were backcrossed to C57BL/6J for 12 generations to generate B6.129P2- Irf7tmiceg mIrf7(Irf7 −/− ).
Trees were generated for 1,000,000 generations, sampling every 100 generations.
Nf2+/− mice were backcrossed for ≥6 generations on a C57Bl/6J genetic background.
They had lived in the forest for 10 generations.
This protocol was repeated for 3 generations (see Fig. 1a).
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