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Observers were asked to first report the perceived shape after looking at it for 5 secs.
The bead suspension was sonicated for 5 secs and allowed to attach to cells for 2 hrs.
Vanilla water was presented for 5 secs followed by lemon water for the same time and this was repeated 10 times at intervals of approximately 60 sec.
PCR cycling was carried out on a Perkin Elmer 9600 thermal cycler and involved 1 cycle of 95°C for 10 mins; 35 cycles of 95°C for 5 secs, 50°C for 20 secs and 60°C for 45 secs; 1 cycle of 60°C for 2 mins.
The sequencing reaction was carried out using the M13 forward primer for 25 cycles (96°C for 10 secs, 50°C for 5 secs and 60°C for 4 minute and hold at 4°C).
If you are hit by a Disarming spell, you're not allowed to shoot a spell at anyone for 5 secs.
Similar(52)
Each odorant was delivered for 5 sec.
Subjects were paced for 5 sec.
PCR amplification was 40 cycles of 94°C for 30 sec, 62°C for 5 sec and 68°C for 60 sec.
Thermocycling conditions were: 95x°C for 5 sec; and 50 cycles: 95x°C for 15 sec; 60°C for 45 sec and finally 1 time 40°C 30 sec.
The PCR amplification conditions were 95°C for 30 sec, 45 cycles of 95°C for 5 sec, 60°C for 20 sec and 40°C for 30 sec for cooling.
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CEO of Professional Science Editing for Scientists @ prosciediting.com