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The chamber was flushed with N2 for 2 hrs.
Then, films were treated in muffle furnace at 400, 500 and 600°C for 2 hrs under air.
Tissue sections were then incubated for 2 hrs at RT.
Prior to stimulation, cells were serum starved for 2 hrs.
Samples were incubated for 2 hrs at 37°C, precipitated with ethanol, and resuspended in H2O.
All samples were incubated at 30°C for 2 hrs with periodical mixing.
This mixure is incubaed for 2 hrs to allow antibody and protein binding.
Glucose levels were measured sequentially for 2 hrs after the meal (Figure S1).
In short, IM-PTEC cells were briefly serum-starved for 2 hrs in DMEM/F12 medium.
Rats remained in the restraining box for 2 hrs at 25°C under dim illumination.
These cells rested for 2 hrs in a 37°C incubator.
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