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We observed that these thermomemory phenotypes mostly lasted for 2 generations.
I can only keep it up for 2 generations.
Xenograft propagated tumors, which were derived initially from pure DCIS tissue, produced tumors in 4 out of 5 mice for 2 generations (Figure 1 and Figure S2).
Lines were crossed sequentially for 2 generations before being thoroughly mixed to produce a single population, which was then maintained as a 14-vial culture (population size = 448).
Mice were then backcrossed for 2 generations onto C57Bl/6 background and then, to facilitate subsequent characterization, intercrossed to obtain homozygote lines.
Worms growing on E. coli OP50 for 2 generations were transferred at the L4 stage to start the lifespan.
Similar(47)
B6 129P2- Irf7tmiceg mice were backcrossed to C57BL/6J for 12 generations to generate B6.129P2- Irf7tmiceg mIrf7(Irf7 −/− ).
Trees were generated for 1,000,000 generations, sampling every 100 generations.
They had lived in the forest for 10 generations.
This protocol was repeated for 3 generations (see Fig. 1a).
Nf2+/− mice were backcrossed for ≥6 generations on a C57Bl/6J genetic background.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com