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The fruit were removed and immediately assessed (Day 1 (Day after Harvest - DAH), or were stored for 2 (Day 3) or 4 days (Day 5) in an ethylene-free room at 20 °C and then assessed.
For deltamethrin, LT50 was longest for 2 day olds with RR50 = 5.33 and shortest for the 10 days old individuals with the RR50 = 3.01.
Arabidopsis thaliana seeds were stratified for 2 day at 4°C in Eppendorf tubes with distilled water.
For deltamethrin, the RR50 was highest for 2 day old and lowest for 10 day old individuals.
For hypocotyl measurements Col-0 seeds were plated on ½ MS plates containing the indicated compounds, incubated at 4°C in the dark for 2 day for stratification and subsequently transferred to long day conditions.
Small mycelial agar plugs were grown out on cellophane membranes on top of PDA at 20°C for 2 day, and then the mycelium was harvested from the cellophane membranes for dsRNA extraction.
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