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The total volume of the reaction mixture (1 ml) was mixed and incubated for 10 second at room temperature.
Following this, the solution was mixed immediately for 10 second then incubated for 10 minutes at room temperature.
The following amplification protocol was used: 95°C for 1 minute (activation), 60 cycles of 95°C for 10 seconds, and 60°C for 10 seconds, followed by 40°C for 10 second final cooling.
If the rat failed to find the platform within 60 seconds, it was picked up and placed on the platform for 10 second then removed and placed under a heat lamp while the platform is moved and the cylinders put in position for the test trial.
Melt the pieces in the microwave for 10 second intervals, stopping to stir, until they are smooth and shiny.
Similar(55)
Wane will doubtless be pleased with his side's mettle if not their game management but, had Hull attacked throughout as they did for 10 second-half minutes, they would have won themselves.
Continue for 30 seconds.
Hold for 15 seconds.
Shake hard for 10 seconds.
Hold both for 10 seconds.
Hold there for 10 seconds.
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