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Tc1 mice have been previously shown to have severe deficits in motor skills in different motor coordination tasks such as the rotarod, static rod and footprint tests [19].
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Similar to the footprint test, baselines scores were collected after one week of training.
Their behavior was assessed by BMS, grid walking and footprint test with and without CNO administration 8 weeks after transplantation.
Rota rod test and footprint test were performed as previously described.
For the footprint test, the fore-toes and the hind-toes were labelled in blue and red, respectively.
The walking footprint test was done on all rats of each experimental group, at the end of pharmacological treatments.
For comparing motor ability and coordination in R6/2 mice and their WT, a footprint test was performed.
The footprint test was used to compare the gait of Tc1 mice with that of WT control mice.
The footprint test is a common qualitative readout that displays the footprint pattern and footprint contacts, potentially highlighting gait abnormalities [ 13].
A footprint test was performed by painting hindlimbs with non-toxic ink and placing mice at one end of an enclosed, dark tunnel on white paper.
For the footprint test, the forelimbs and hindlimbs of mice were painted as blue and red with non-toxic ink respectively.
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