Footprint maps were produced by a simple model based on the analytical solution of the Eulerian advection diffusion equation.
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The human footprint maps the continuum of human influence over terrestrial area and is similar to the 'ecological footprint', which estimates the amount of area necessary to support the consumption of one or more persons [33].
Each time a footprint mapped to a nucleotide position, we incremented its occupancy by the tag count of the footprint.
Individual LysC DsRed+ cells were identified using automatic "find objects use intensity" and "colour the object" functions in Volocity for all the time points to generate a "footprint map".
The proportion of ribosome footprints mapping inside predicted uORFs was calculated for each gene expressed in each stage.
(B ) Proportion of ribosome footprints mapping inside or outside predicted uORFs in the HMM-defined 5′ leaders.
Our data clearly shows a difference between footprints mapping to the 5' leader and CDS, as opposed to noncoding RNA derived fragments (rRNAs, tRNAs).
Our results demonstrate a lack of significant difference between the expected and observed proportion of footprints mapping to exonic TA sites (p = 0.076, two-tailed two-proportion z-test), providing minimal evidence of such enrichment.
Footprint enrichment is specific to 5′ leaders and not due to non-specific background since this would result in an increase of footprints mapping evenly along the length of the transcript, including the 3′ UTR, and not just the 5′ leader.
The disease and Nitrogen-footprint maps have compelling similarities (Fig. 4) which the GWR test confirms.
