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Accordingly, footprints that are more aligned have a smaller footprint alignment value.
Footprint alignment.
Footprint alignment values also decreased in flies deprived of sensory feedback.
At slow speeds (<20 mm/s), footprint alignment values were relatively large while at fast speeds (>34 mm/s) these values were much smaller, suggesting that footprint alignment is highly constrained.
In support of this possibility, the tripod index increases with lower footprint alignment values (data not shown).
Notably, at intermediate speeds (between 20 and 34 mm/s), there was no correlation between speed and footprint alignment.
Similar(46)
In addition, the RNAz server (Vienna RNA server, http://rna.tbi.univie.ac.at) was used to identify thermodynamically stable and evolutionarily conserved RNA secondary structures in the footprint alignments.
Ribosome-protected footprint alignments were mapped to their estimated P-sites as follows: 12 nt were pruned from each end of the alignment, leaving a fragment n nt long (where n = l − 2 × 12).
The complete phylogenetic footprinting alignments of the different OXPHOS polypeptides, highlighting the conserved regions, can be found in additional files (Core Promoter Additional files 4, 5, 6, 7, 8).
We describe the use of EvoPrinter and cis-Decoder, a suite of interrelated phylogenetic footprinting and alignment programs, to characterize highly conserved sequences that are shared among co-regulating enhancers.
Structural alignments were computed by the VAST algorithm [ 16] and only those structures which had more than 80% mutual overlap between the VAST alignment footprint and CDD footprint were considered in the analysis.
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