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Allograft bone particles, Ti and Tci, were manually impacted with a sliding thread (diameter 1.8 mm), with a sliding weight of 9.8 g ending against a stopper at the foot of the thread.
The foot of the thread was inserted into the upper lumen of the bone chamber (on top of the grafts, acting as an impactor) containing the graft particles during impaction in the rest of the lumen of the bone chamber.
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Grasp the small handle on the side of the machine to raise the presser foot so that the thread can pass through the threading points on the machine.
Each byssal thread and adhesive plaque is formed in an elegant injection molding process that begins with secretion of liquid protein precursors into a cavity defined by the mussel foot, followed rapidly by solidification of the thread and plaque over several minutes.
Cut about one foot of thread, tie a knot in the lower end and thread your needle.
Hold the free end of the thread and, keeping fingers clear of all moving parts, press the foot pedal or knee lever.
Thread a needle with a long piece of the thread.
Tie off the end of the thread.
Cut the opposite side of the thread.
Leave the end of the thread outside.
Tie the ends of the thread together.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com