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As a typical example, the synthesis of mPEG5-PLA15 was carried out as follows: to remove any trace of water, the starting materials (5 g of mPEG5 and 15 g ofd,l-dilactide) were each dissolved in 100 mL toluene in a round-bottomed flask.
pBF3 was constructed as follows to remove unnecessary elements of pBF1: pBF1 was first cut with AvrII, and Acc65I, and the ends filled in with DNA Polymerase I, Large (Klenow) Fragment (New England Biolabs) to generate blunt ends for ligation.
Prior to addition of I-PFO*, cells were washed as follows to remove surface-bound lipoproteins or HPCD: three rapid washes with buffer A at room temperature, followed by two 10-min washes with the ice-cold buffer A in a 4°C cold room.
GAG liberation and purification was conducted as described for guanidine-extracted PG with the exception of an additional purification step as follows to remove contaminating DNA in the tissue extracts: GAG-ethanol precipitates were solublized in 500 μl of dH2O, adjusted to a final concentration of 10% trichloroacetic acid, 0.01% BSA and incubated on ice for 1 hour.
Similar(56)
Another spade finesse was taken, and the ace followed to remove West's king.
Finally, in the interests of both fairness and transparency Treasury recommends providing a "clear offramp" for SIFIs to follow to remove a designation, including establishing such a process as part of its annual SIFI reevaluations.
Once the design of upgrade is authorized, changes to the system can be made at suitable times, after which a proper release to service process must also be followed to remove any uncertainty during operation.
Then, a typical wet clean process can be followed to remove those residual fragments as stated above.
Two more operations followed to remove tumors, but her disease continued to progress.
Several washing steps followed to remove SDS.
S1 nuclease treatment followed to remove single stranded DNA and single strand overhangs on renaturated double stranded DNA.
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