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To obtain ∼1.0×107 clusters for one lane, the general procedure as described in the standard recipe (Illumina) was performed as follows: template hybridization, isothermal amplification, linearization, blocking, denaturation, and hybridization of the sequencing primer (Illumina).
Finally, after validating on an Agilent Technologies 2100 Bioanalyzer using the Agilent DNA 1000 chip kit, the cDNA library was sequenced on a PE flow cell using Illumina Genome Analyzer HiSeq 2000, and the workflow was as follows: template hybridization, isothermal amplification, linearization, blocking, sequencing primer hybridization, and sequencing on the sequencer for Read 1.
Polymerase chain reaction conditions were as follows: template DNA (100 ng) was amplified in a total volume of 50 μl containing 150 μ M of each dNTP, 1.5 m M MgCl2, 15 pmol of each primer, and 1.25 U of HotStar Taq polymerase (Qiagen, Hilden, Germany).
Finally, after validating on an Agilent Technologies 2100 Bioanalyzer using the Agilent DNA 1000 chip kit, the cDNA library was sequenced on a PE flow cell using Illumina Genome Analyzer IIx, and the workflow was as follows: template hybridization, isothermal amplification, linearization, blocking, sequencing primer hybridization, and sequencing on the sequencer for Read 1.
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Following template reactions, templated libraries were assessed for quality using the Ion Sphere Quality Control Kit (Catalog # 4468656) and Qubit fluorometer (Life Technologies).
Following template library preparation, 48 accessions, each containing a unique 4-bp MID tag, were pooled/lane on the Illumina GAIIx.
And to enable us to create new words and phrases, we adopt a word-processing word to express a pattern or gauge to follow: templates.
Measure the main girl's head to get the correct circumference on a template and follow the template with the evergreen branches.
Every so often an article appears that follows a template so ingrained it should be a madlib.
While TWPGAT13 slavishly follows the template set down by the first Tiger Woods games more than a decade ago, there are some updates and changes.
Contemporary politics follows the template of Vladimir Lenin's basic precept: kto-kogo, literally "who-whom," meaning who does what to whom.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com