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AF0-8497; Phenomenex, USA) and eluted at a flow rate of 0.6 ml per min with a gradient of 0.1% formic acid in water (mobile phase A) and methanol (mobile phase B) as follows (minute, percentage of B reached at that minute): 0 min, 2% B; 1 min, 2% B; 8 min, 100% B; 9.3 min, 100% B; 9.5 min, 2% B; and 11.2 min, 2% B for equilibration.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com