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This is its fifth discovery in the region, and follows disruption at Tullow sites in Kenya last month after protests by local residents demanding jobs and other benefits.
In yeast, a defect in respiratory efficiency was detected that follows disruption of iron-sulphur cluster maturation (Muhlenhoff et al., 2002), whereas iron-sulphur cluster deficit was described to precede the first evidence of a cardiac dysfunction in mouse (Martelli et al., 2012).
Total RNA was isolated as previously described [ 7] but with modified lysis conditions for PCC strains, Anabaena and Nostoc as follows: Disruption by adding an equal volume of glass beads, 33.3 μl 20 % SDS solution and 583 μl acidic phenol to 500 μl concentrated cell solution, following several cycles of vigorous agitation, freezing in liquid nitrogen and thawing in a water bath.
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The increase in measles cases follows disruptions to social services, many of them caused by the unrest in 2011.
Following disruption, cellular debris was removed by centrifugation at 11,500×g for 20 minutes at 4°C.
Following disruption by sonication the lysate was centrifuged in at Ti45 rotor at 40 krpm for 45 minutes at 4°C.
Following disruption is a cascade of declines resulting in secondary pathologies.
Following disruption of the endothelium, sub-endothelial cell layers are exposed.
Following disruption of the first allele, spontaneous recombination events gave rise to Arg+, Ura+, His− segregants in which one allele is disrupted by the ura3-ARG4-ura3 marker and the other one by the recombined URA3 marker.
Following disruption with scalpels, tissue homogenates were incubated at 37°C in digestion buffer (Ham's F12/DMEM, 2 mg/mL collagenase A (Roche, Indianapolis, IN, USA), 100 U/ml hyaluronidase (Sigma-Aldrich, St . Louis MO USA)).
There are many examples where increasing frequencies and severities of droughts and floods appear to follow disruptions to the water-cycle (Millán 2014; Hirsch and Archfield 2015; Erfanian et al. 2017).
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