The injected sample volume was 10 μL with the HPLC settings as follows: column Supelcosil™ LC-NH2, 5 μm (25 cm × 4.6 mm), mobile phase of 0.75% acetonitrile in water, flow rate of 1.0 ml/min, run time of 10 min, column temperature of 35 °C, and refractive index detection (Agilent 1200 model).
Analysis conditions were as follows: column temperature of 30°C; mobile phase of methanol and 0.5% acetic acid [dissolved in redistilled water, 45:55 (v/v)]; and flow rate of 0.8 mL min−1.
The positive control plate format was as follows: Column 1, potassium dichromate, a cytotoxic agent, ranged from 2.8 nM to 92 µM; column 2, staurosporine, a caspase 3/7 inducer, ranged from 2.8 nM to 92 µM; column 3, DMSO only; and columns 4, 92 µM potassium dichromate.
The chromatographic conditions are described as follows: column: Pharmacia column (1.6 × 90 cm), gel: Sephacryl S-400-HR (Sigma Chemical Co., USA), mobile phase: 0.15 M NaCl, sample concentration: 10 mg/mL, and flow rate: 0.5 mL/mg, 2.5 mL/min.
The GC conditions (column 1) were as follows: column temperature program, from 105°C (hold 2 min) to 300°C at 20°C/min (hold 5 min); carrier gas, helium (Linde, Prague, Czech Republic) with a constant flow of 1.5 mL/min; injection temperature, 275°C; injection volume, 1 μL using pulsed splitless injection mode (splitless time, 2 min).
HPLC conditions were as follows: column, ODS (TSK-GEL 80TS, 250 × 4.6 mm i.d., TOSOH, Tokyo, Japan); eluant, (A) 0.05 M AcONH4 (pH 3.6) (B) 100% CH3CN (a linear gradient of 90% A and 10% B, which changed over 60 min to 0% A and 100%, B was used, followed by 100% B for a further 20 min); temperature, 40°C; flow rate, 1.0 mL/min.
This follows columns by the Mail's chief political thinker, Simon Heffer, accusing Germany of trying to found a "Fourth Reich".
Binary tables This approach [1, 2] follows column-oriented database schema organization and defines a two-column table for each property containing the subject and object, resulting in a set of tables each of which are ordered by the subject.
Again, these suggested account will reside in the Who to follow column on Twitter's right-hand pane.
Following column washes, bound cDNA was eluted according to manufacturer's instructions.
Following column purification, samples were eluted into 10 μL buffer TE (10 mM Tris, pH 8.0; 1 mM EDTA).
