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Following this initial step, sterile medium was then pumped through the MRD at a constant flow rate.
Following this initial step, administer a GnRH agonist (GnRHa) therapy for 3 months, during which time the thickness of the cyst wall significantly decreases, with atrophy and reduction in stromal vascularisation of the cyst [6].
Following this initial step, members of the research team met to discuss the emerging concepts and through consensus, an initial coding scheme of the predominant themes was developed.
Following this initial step, the time-dependent measurements were performed by incubating equimolar amounts of NE and active A1AT (1nM each) in a 1 ml polystyrene cuvette with a 1 cm pathlength at 23°C.
Following this initial step, there is elongation and modification of the oligosaccharides. 2 Mucin-type O-glycans can be further subdivided into eight core structures depending on the second sugar(s) and/or binding position resulting in at least 50 different types.
Following this initial step, the time-dependent measurements were performed by incubating equimolar amounts of PR3 and active A1AT (1nM each) in a black opaque polypropylene low binding plate (Sigma, UK) at 23°C with a well volume of 150 μL.
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Second, following this initial merge step, we then considered all hits within the node's subtree and between the subtree and all outgroup genes in descending order of bit score (in either source-target gene direction).
It can therefore be employed in capacity building, following the initial step of research priority setting [ 9].
In this work a detailed transformation network of competitive reaction pathways following the initial steps of oxidation has been studied theoretically using density functional theory (DFT).
PCR amplification was carried out under the following conditions: initial step at 95 °C for 5 min; followed by 50 cycles at 95 °C for 10 s, Ta of the respective primer set for 30 s and 72 °C for 10 s (touchdown 1 °C, 7 cycles); denaturation step at 95 °C for 1 min followed by a hybridization step at 40 °C for 1 min.
The RT-PCR was performed using Superscript III one-step RT-PCR systems with Platinum Taq (Invitrogen, Carlsbad, California) under the following thermocycler conditions: initial step of 94°C for 2 minutes, followed by 40 cycles of 94°C for 15 seconds, 60°C for 30 seconds, and 68°C for 1 minute, with a final extension step of 68°C for 7 minutes.
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