Sentence examples for following the specified protocol from inspiring English sources

The cells were washed subsequently with PBS and subjected to MACS to purify the neural progenitors following the specified protocol (Miltenyi Biotec).

The Sca4 antigen gene (sca4) was amplified by using the primer pairs D1f and D928r, D767f and D1390r, D1219f and D1876r, and D1738f and D2482r, following the specified protocol (19 ).

Those who are not using the drugs according to the study treatment protocol might have their treatment modified at this time following the specified treatment protocol described in the intervention section.

The genomic DNA of B. xenovorans was isolated from the B. xenovorans LB400 cells by following the manufacturer's specified protocol for the DNA Mini Kit.

Probe labeling and hybridization were carried out following the manufacturer's specified protocols.

The PCR products were purified and sequenced following the same protocols specified above.

3' and 5' adapters were ligated to the small RNAs and constructs amplified following RT-PCR following the conditions specified in the small RNA kit (FC-102-1009, Illumina) protocol.

When such an entity exists, and the Office of Risk Management has reviewed and approved the group's training procedures and flight safety protocols, the group can apply to conduct flights following the process specified above.

For the samples subjected to RNA-Seq, we isolated 5 μg of total RNA from each sample and prepared them for high-throughput sequencing following the standard mRNA protocol by Illumina, unless specified otherwise.

Twenty-two percent (22/98) of all samples met the required DNA amount as specified by the GeneRead protocol, and were added at 20 ng per primer pool (4 pools to cover BRCA1 and BRCA2) following the standard protocol (25 PCR cycles).

RNA was extracted using TRIzol (Invitrogen) following the supplied protocol.