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Following the sorting and culturing of 4 individual subsets, the only cell subset, which gave rise to adipose-derived and expanded-mesenchymal stem cells in our study, was originated from the conventional ADSCs subset.
Following the sorting of reads to each individual sample, the 12 bp barcode on the 5′ end was trimmed and the reads were imported into the CLC Genomics Workbench v9.0 (Qiagen, Boston, MA, USA).
Following the sorting of individual reads by barcode, sequences are aligned to the appropriate reference for analysis.
Following the sorting of CCS reads by forward reverse barcode pairs, sorted reads were aligned back to the reference sequence using Bowtie2.
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Sorted cell populations were defined as the following: neurons (NeuN+/CD11b−/GFAP or GLT-1−), microglia (CD11b+/NeuN−/GFAP or GLT-1−) and astrocytes (GFAP or GLT-1+/CD11b−/NeuN−). Immediately following the sort, the cells were pelleted and resuspended in Tri Reagent for RNA isolation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com