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This study uses information from 35 permanent forest plots to generate equations for predicting ACD from ALS data, 32 of which were established as part of New Zealand's Land Use and Carbon Analysis System (LUCAS), while 3 others were established following the same protocol for other purposes.
For RNA-T cells, PBMCs were electroporated with OKT3-28BB RNandnd re-suspended at a concentration of 2 × 106/mL in culture medium supplemented with 300 IU/mL IL-2; the cells were then cultured and maintained following the same protocol for the OKT3/IL-2 T cells.
Patients were treated following the same protocol for both DH and CH.
Each dog underwent an echocardiographic examination, following the same protocol for strain and torsion measurements as described above.
The TGF-β131 cell line was generated by transfecting the pcDNA-GS-TGF-β131 construct into CHO-lec3.2.8.1 CHO-lec3.2.8.1pofecellsne 2000 (Invitrogen) following the same protocol for TGF-β1.
The program was standardized, following the same protocol for lymphatic drainage to the anterior trunk, posterior trunk, and affected arm, always mowing fluid from the affected side toward the unimpaired side, after lymphatic drainage and before either the short-stretch bandages or the Kinesiology Taping application.
Similar(53)
For those who cease driving, annual follow-up continues following the same protocol as for all participants, excluding instruments and records relevant only to drivers (e.g., vehicle inspection, driving records) (Fig. 1).
The specimens were then prepared for SBS testing following the same protocol described for the preparation of enamel specimens, except for the acid-etching step which was carried out using 10% hydrofluoric acid for 90 s (Condac Porcelana, FGM).
Libraries for these spikes were produced following the same protocol as for the pooled barcoded experimental samples, resulting in one library for spiking the sequencing of smaller size range amplicons (~130 base spike) and one library for spiking the sequencing of larger size range amplicons (~280 base spike).
RNA isolation from Calu-3 2B4 cells infected with MERS-CoV Eng 1 was performed following the same protocol used for MERS-CoV SA 1-infected Calu-3 samples and used for subsequent hybridization to Agilent 8 × 60K human arrays.
Eggs were brought to our insectary for hatching and the resulting larvae reared following the same protocol as for the isogenic strain experiment.
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