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The observed lack of processing following sequestration is consistent with a general cellular pathway rather than conventional vector trafficking.
As endocytosed EGFR were not sorted to lysosomes following sequestration in early endosomes in RBE cells, we hypothesized that non-degraded EGFR might undergo recycling back to the cell membrane after this trafficking block.
Mechanistically, we uncovered that premature senescence of lung fibroblasts induced by oxidative stress occurred through activation of an ataxia telangiectasia-mutated (ATM /p53-depedent pATM /p53-depedentsequestration of the catalytic subunit of pathway phosphatase 2A (PP2A-C), an inhibitor ofollowing caveolin-1 into caveolar membranesequestration
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For instance, we do not yet know what happens to pri-let-7 following its sequestration into the nucleolus by methylated LIN28A or LIN28B.
Anaemia of chronic disease following the sequestration of iron in the reticuloendothelial system has been recognized as a clinically important entity for many decades [ 49].
To determine the intracellular fate of NP1 following endosomal sequestration we took advantage of the electron dense core of NP1-MNP to investigate intracellular localization using transmission electron microscopy (TEM).
To illustrate the method, we used estimation of soil C stocks in mixed-species tree plantings and pastures to estimate carbon sequestration following reforestation.
We conclude that following intradermal infection, sequestration of Bartonella spp. in tissues may limit diagnostic detection of these bacteria in dog blood samples.
It would be interesting to examine p53 sequestration following IL-17-induced synoviolin upregulation, and the ubiquitination status of p53 at later timepoints.
Similar assays for the irreversibility of PML-ND sequestration following heat shock revealed sequestered PML-NDs on 90±6% of DsRed1-E5-ataxin-1 IBs and 93±4% of GFP-ataxin-1 IBs of over 1 µm in diameter.
We did not see abnormal TDP-43 sequestration following PGRN knock down or mutant TDP-43 overexpression, consistent with previous results in other animal models [26], [30], [31], [32].
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