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The samples were then warmed to room temperature and incubated with phosphate-buffered saline supplemented with 0.1% v/v Triton X-100 (PBS-T) for 1 h at room temperature following sequential washes (5 min) in 75% methanol/25% PBS-T, 50% methanol/50% PBS-T and 25% methanol 75% PBS-T.
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We performed the following sequential action: (1) the cells detached with trypsin and EDTA; (2) centrifugation at 750×g for 5 min; (3) washing the pellet cells with PBS containing calcium; (4) cells were re-suspended in 100 μl binding buffer; (5) cells were mixed with 2 μl annexin V-FITC; and at the end, (6) 2 μl PI was added to mixture and the flow cytometric analysis was done.
To investigate graft survival and rejection following sequential bilateral corneal transplantation.
*Denotes significant deviation from HWE following sequential Bonferroni correction.
Two contrasts remained significant following sequential Bonferroni correction (Table 6).
The resulting solutions, following sequential dilution, were analysed by HPLC-MS.
DGF rates were increased in the second implant following sequential transplantation (p = 0.05).
Two of the tests remained significant following sequential Bonferroni correction (Table 6).
The computational processes involved can be described in the following sequential steps.
Major vessels can readily be identified by following sequential 3-mm cuts.
Significant test results (following sequential Bonferroni correction) are indicated in boldface.
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