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Data were subjected to Student's t-test following quantification.
All mapped sequences with a copy number equal to or greater than two were used for the following quantification procedure.
Morpholinos were resuspended in RNase-free water and quantified using a NanoDrop ND-1000 (Thermo Scientifollowingowing quantification instructions.
The resultant color images used in the following quantification analysis were in a 1,280 × 1,024 pixel RGB format with a 64-bit resolution.
Polymerase chain reaction (PCR) introduced sample-specific indexes to the library, and following quantification this was hybridized to the custom-capture probes.
The incision of the AP site by various amounts of APE1 was assessed by comparing the levels of DNA found in the intact or cleaved bands following quantification.
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The following quantifications were performed: soil microbial populations by soil dilution plate technique on selective and semi-selective culture media; microbial respiration and microbial biomass by chloroform fumigation-extraction; microbial activity by fluorescein diacetate hydrolysis; and fatty acid methyl ester (FAME) profiles.
Specifically, we added the following quantifications: a) Figure 1 figure supplement 3-4: Quantificatiof of ERK variability when the paracrine signaling length-scale is manipulated; b) Figure 2 was divided in to Figure 2 and Figure 3.
Illumina libraries were clustered for sequencing immediately following sample quantification according to manufacturers recomendations (Illumina).
An adult-like pattern of responses was also observed following the quantification of multifunctional CD4+ T cells secreting 2 3 distinct Th1 cytokines.
SDS sample buffer: 2 ml Glycerol, 2 ml SDS 20%, 2.4 ml 0.5 Tris-HCL pH 6.8, 2.6 ml ddw, and 1 ml beta-mercaptoethanol. SDS sample buffer was added and following protein quantification using the Bradford reagent method all samples were equalize by tris-glycerol, boiled for 5 minutes and stored at −20°C. 10 ug of protein were loaded to a 12% gel and were transferred to a nitrocellulose membrane.
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