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Baking immediately following plating is now widely used to mitigate whiskering, but this effect is routinely attributed to stress relief.
To prevent fibroblast proliferation, 100 mM 5-bromo-2-deoyuridine (BrdU) was added to the cardiomyocyte media for 48 h following plating, at which point media were replaced.
PX-866 was added to the cultures the day following plating.
On day 2 following plating, non-adherent cells were removed from the culture.
Invasion was measured at 48 hours following plating using an MTT readout system.
Washing of unattached NRVMs within 6 8 hours following plating ensured minimum NRVM attachment on top of the CPCs.
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In this study, after the final follow-up, refracture occurred in one female obese patient who fell following plate removal.
The reported incidence of refracture following plate removal ranged from 0 to 8% [2, 22].
Both plates are slowly migrating north, but the weight of the Himalayas slows the leading plate, forcing the following plate to dive under the leader.
The interaction of the shed wakes of plates in a cascade with each following plate is investigated in a water tunnel and shown to provide an explanation for an observed very powerful aerodynamic noise source.
Open image in new window Fig. 3 Post-operative X-ray following plate removal: maintained reduction in pubic symphysis with bone bridge and the closure of both triradiate cartilages.
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