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For automated selection following parameter thresholds of colonies were defined: area: ∅>400 µm; sphericity 1 0.8; convexity: 0.8 1; elongation: 1 5; shape factor: 0.8.
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Here I report a preliminary simulation conducted using the following parameter values: threshold hysteresis, 30 min; supra- and sub-threshold Psw, 0.02062; suprathreshold Pws, initial 0.11715, final 0.00554; subthreshold Pws, initial 0.11593, final 0.00416.
We retrieved the potentially active compounds from CVDHD with the following parameters: the thresholds of docking score, the top percentage of rank of docking score were set to 7.0 and 0.10, respectively.
Using a high-speed infrared camera (25 frames per second), the embryos were tracked in the dark for 10 min with the following parameters: inactivity threshold of 6 mm/s, detection threshold of 25 mm/s, and activity burst threshold of 30 mm/s.
All data were processed by local XCMS with the following parameters: signal/noise threshold = 3, ppm = 10, peak width = (20, 50), and prefilter = (3, 1000).
SICER_v1.1 was downloaded from http://home.gwu.edu/~wpeng/Software.htm, and the script SICER.sh was run with the following parameters: redundancy threshold = 1, effective genome fraction = 0.81, window size = 2000, gap size = 6000, FDR = 0.001, E-value=0.1.
Using Haploview 4.2 (with the following parameters: r threshold: 0.8; HW p value cutoff: 0.05; minimum genotype: 95%; and minimum minor allele freq.: 0.05), 4 tag SNPs (rs969885, rs12483428, rs987195, and rs4817027) were selected, and their association with epilepsy was investigated.
PISCES (Wang and Dunbrack, 2003) has been used with the following parameters: sequence identity threshold 20%, resolution threshold 2 Å and R-factor threshold 0.25.
The blastp algorithm used gapped alignment with the following parameter: expectation value 10-300), open gap (−1), extend gap (−1), threshold for extending hits (11) and word size (3).
The limiter for low temperature operation having the same initial losses could be designed with the following parameters: 4 V threshold voltage and 8 dB attenuation in the limiting regime.
Interpretation of the results was carried out with the CGH Analytics® 3.5.14 software, set with the following parameters: ADM-2, threshold: 6.0, window: 0.5 Mb, cut-off: 0.25.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com