Using the following minimum angle distance, we can identify one-to-one shared orthologs.
Three quantitative real-time PCR reactions were performed during this study following the Minimum Information for publication of Quantitative real-time PCR Experiments (MIQE) guidelines [24], and further information on data analysis and methodology is summarized in the supplementary tables S1 to S4.
Gene expression studies were performed following MIQE (Minimum Information for publication of Quantitative Real-Time PCR Experiments) guidelines [ 30].
The microarray experiments were conducted by following the minimum information about a microarray experiment (MIAME) guidelines [ 56].
Microarray production, hybridization, and data analysis were performed following the minimum information about a microarray experiment (MIAME) guidelines for international standardization and quality control of microarray experiments [ 46].
All datasets in this study were published within the past 5 years (2004-2009) and following the Minimum Information about a Microarray Experiment (MIAME) guidelines, including 49 datasets, with 3,832 samples containing 16, 15, 11, and 7 datasets from colon, liver, lung and thyroid cancer, respectively (Additional file 1: Table S2).
An example is the Gene Expression Ombinus (GEO [ 8, 9]); GEO (similarly to ArrayExpress [ 23]) is a data repository for microarray and NGS data that requires the data producers to submit data following the Minimum Information About a Microarray Experiment (MIAME) guidelines [ 24].
As this operation also shifts edges, the following minimum-filtering reverses the edge-shifting.
2 µl of resultant fscDNA was used for RT-qPCR, following the MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines [39].
But the peek was carefully scripted, following her maxim of "minimum information given with maximum politeness".
