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Ex vivo isolated CD8+CD28− T cells contained more damaged DNA than their CD8+CD28+ counterparts following isolation, but before stimulation.
Therefore, the 95%% ethanol extract was chosen for the following isolation.
Purification of amplified DNA product, following isolation, was done in a mandatory basis prior to sequencing of that amplified DNA.
At present, a quantitative measurement of the modifications to elastin following isolation from other extracellular matrix constituents has not been reported.
Other examples, such as grafts of skin to treat severe burns, entail transplantation of stem cells within organized tissue rather than following isolation.
One study assayed SNc dopaminergic neurons only, following isolation by laser capture microscopy (LCM) [13].
After blocking, PBMC without any prior manipulation following isolation from peripheral blood were added to wells.
Human islets were received in our laboratory 3.1±1.4 days following isolation.
Adenovirus infections were initiated immediately following isolation at 500 multiplicity of infection (MOI).
The observation of cellular memory, or retention of environmental properties following isolation, has been reported previously.
Immediately following isolation, MNC from BM and PB were processed for FACS analysis.
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