Briefly, the results show that internalized WGA, following internalization via clathrin-coated vesicles (Fig. 1a insert) and possibly other mechanisms as well, is rapidly transported to the Golgi apparatus, and induces dramatic Golgi reorganizations.
This could be due to the fact that NPs are in vesicles together following internalization.
It is generally known that these two isotopes have different fates following internalization.
With the special non-clathrin-dependent endocytosis, TAS/DOX/pDNA NPs presented higher cellular uptake and mainly distributed in ER and Golgi rather than lysosomes following internalization.
Importantly, following internalization by lung cancer cells, the reducible DOX-encapsulated SS-NPs achieved higher cytotoxicity than the non-reducible thioester NPs whereas blank nanoparticles were non-cytotoxic.
We thus propose that LST-4 constitutes another member of the evolutionarily conserved mechanism that drives early phagosome maturation following internalization of apoptotic cell corpses.
Alternatively, some APP is processed by β-secretase in the Golgi or in late endosomes following internalization from the cell membrane.
Using cAMP FRET biosensors, we show that levels of cAMP rise quickly at the nascent phagocytic cup and return to baseline following internalization of the particle.
These results suggest an alternative, more efficient macrophage uptake mechanism for Francisella that requires exposure of a specific bacterial surface structure(s) but results in increased cell death following internalization of live bacteria.
In order to investigate which intracellular compartments Kb molecules traffic through following internalization from the DC surface, spleen-derived DCs from all transgenic mice were initially surface-labeled at 4°C with Kb-specific mAbs.
Although CHL1/Hsc70/αSGT complexes are formed at the plasma membrane, they can redistribute to synaptic vesicles following internalization of CHL1 to these organelles [7], an observation that would explain lower levels of Hsc70 and αSGT in synaptic vesicles from CHL1−/− mice.
