Intracellular staining was done following fixation and permeabilization of cells.
The objective of this study was to evaluate changes of the lumbar vertebral column following fixation.
After colony-formation, the number of colonies was counted following fixation with a 10% formaldehyde neutral buffer solution, and subsequent staining with 2% rhodamine.
Open image in new window Fig. 5 Pelvis following fixation failure.
A good outcome can be achieved for these injuries following fixation.
All other technical steps following fixation were similar to those described for in vitro samples.
Antigen retrieval was performed following fixation of cells with 4% paraformaldehyde.
As shown in Figure 5, fluorescence was maintained following fixation and showed only modest decay during a two-week incubation.
Whole brain weight (±0.001 g) was obtained from dissected brains following fixation (see [23] for further details).
The cells were then subjected to intracellular cytokine staining following fixation and permeabilization with cytofix and cytoperm buffer [48].
All measures were performed on live animals, except for phallus length in silvery mole-rats which was measured following fixation.
