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Rats were randomly divided in groups formed by 4 6 animals each, and underwent the following experimental protocols.
All animal experiments were conducted following experimental protocols previously approved by the Institutional Ethics Committee on Experimental Animals, in full compliance with Spanish and European laws and regulations.
Eight animals from each group were evaluated 90 days after myocardial infarction induction (105 days after diabetes induction) to perform the following experimental protocols.
After 30 min of stabilization, hearts from SHR were assigned to the following experimental protocols: nonischemic control hearts (NIC; n = 8): hearts were perfused for 135 min without any treatment; ischemic control hearts (IC; n = 10): hearts were subjected to 45 min of normothermic global ischemia followed by 1 hour of reperfusion.
In an additional study, the atrial muscles (n = 6/group) were subjected to the following experimental protocols: (i) aerobic perfusion for 240 minutes; (ii) aerobic perfusion for 240 minutes with the caspase inhibitor z.DEVD.FMK (70 nM); (iii) 90 minutes simulated ischaemia followed by 120 minutes reoxygenation; and (iv) simulated ischaemia/reoxygenation with 70 nM z.DEVD.FMK (70 nM).
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In order to measure the half-life of endogeneous NICD, the following experimental protocol is followed: 9 embryos are dissected along their midlines with contralateral halves placed in two pools (A and B).
The isolated jejunum (6 cm) was subjected to 30 minutes ischemia followed by a median reperfusion time of 128 minutes (range: 0 210 minutes) according to the following experimental protocol.
The following experimental protocol was approved by the Institutional Animal Care and Use Committee of the Université de la Méditerranée.
To study the effects of AG on the Stx2-treated pregnant rats, the following experimental protocol was used.
The following experimental protocol was approved by The Ohio State University, Laboratory Animal Care and Use Committee.
Accordingly, the following experimental protocol was set up (Fig 4B).
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